Translation in a cell‐free system, and in Xenopus oocytes, of mRNA which specify a cadmium‐binding protein in Nereis diversicolor (annelida, polychaeta)
Identifieur interne : 000B75 ( Main/Exploration ); précédent : 000B74; suivant : 000B76Translation in a cell‐free system, and in Xenopus oocytes, of mRNA which specify a cadmium‐binding protein in Nereis diversicolor (annelida, polychaeta)
Auteurs : Djamal Eddine Benouareth [France] ; Nicole Dhainaut‐Courtois [France] ; Eliane Porchet‐Hennere [France] ; Jean‐Jacques Curgy [France]Source :
- Biology of the Cell [ 0248-4900 ] ; 1989.
English descriptors
- KwdEn :
Abstract
The presence in the marine worm Nereis diversicolor of a low molecular mass protein with the capacity to bind cadmium has been previously demonstrated [5]. Poly(A)+‐mRNA were extracted from coelomocytes of Nereis diversicolor and were translated either in vitro, using a rabbit reticulocyte lysate, or in vivo into Xenopus laevis oocytes. Analysis of synthesized polypeptides by enzyme‐linked immunosorbent assay (ELISA) and by Western blotting, using a specific monoclonal anti‐MP II antibody, showed that this metalloprotein was translated both in in vitro and in vivo translation systems, with an apparent molecular mass of 11–13 kDa. Two other products, with 26.5 and 28 kDa molecular mass, cross‐reacted with the monoclonal anti‐MP II antibodies. The present work confirms that coelomocytes are sites of important synthesis of MP II‐mRNA.
Url:
DOI: 10.1111/j.1768-322X.1989.tb00859.x
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Translation in a cell‐free system, and in Xenopus oocytes, of mRNA which specify a cadmium‐binding protein in Nereis diversicolor (annelida, polychaeta)</title><author><name sortKey="Benouareth, Djamal Eddine" sort="Benouareth, Djamal Eddine" uniqKey="Benouareth D" first="Djamal Eddine" last="Benouareth">Djamal Eddine Benouareth</name></author><author><name sortKey="Dhainaut Ourtois, Nicole" sort="Dhainaut Ourtois, Nicole" uniqKey="Dhainaut Ourtois N" first="Nicole" last="Dhainaut‐Courtois">Nicole Dhainaut‐Courtois</name></author><author><name sortKey="Porchet Ennere, Eliane" sort="Porchet Ennere, Eliane" uniqKey="Porchet Ennere E" first="Eliane" last="Porchet‐Hennere">Eliane Porchet‐Hennere</name></author><author><name sortKey="Curgy, Jean Acques" sort="Curgy, Jean Acques" uniqKey="Curgy J" first="Jean‐Jacques" last="Curgy">Jean‐Jacques Curgy</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:672C54E5C41ADEBAA864BDF4D175704F7C08B5B0</idno><date when="1989">1989</date><idno type="doi">10.1111/j.1768-322X.1989.tb00859.x</idno><idno type="url">https://api.istex.fr/document/672C54E5C41ADEBAA864BDF4D175704F7C08B5B0/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">000569</idno><idno type="wicri:Area/Main/Curation">000569</idno><idno type="wicri:Area/Main/Exploration">000B75</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Exploration">000B75</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Translation in a cell‐free system, and in Xenopus oocytes, of mRNA which specify a cadmium‐binding protein in Nereis diversicolor (annelida, polychaeta)</title><author><name sortKey="Benouareth, Djamal Eddine" sort="Benouareth, Djamal Eddine" uniqKey="Benouareth D" first="Djamal Eddine" last="Benouareth">Djamal Eddine Benouareth</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Endocrinologie des Invertébrés, CNRS UA 148, Université de Lille‐Flandres‐Artois, F‐59655 Villeneuve‐d'Ascq Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Hauts-de-France</region><region type="old region" nuts="2">Nord-Pas-de-Calais</region><settlement type="city">Villeneuve‐d'Ascq</settlement></placeName></affiliation></author><author><name sortKey="Dhainaut Ourtois, Nicole" sort="Dhainaut Ourtois, Nicole" uniqKey="Dhainaut Ourtois N" first="Nicole" last="Dhainaut‐Courtois">Nicole Dhainaut‐Courtois</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Endocrinologie des Invertébrés, CNRS UA 148, Université de Lille‐Flandres‐Artois, F‐59655 Villeneuve‐d'Ascq Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Hauts-de-France</region><region type="old region" nuts="2">Nord-Pas-de-Calais</region><settlement type="city">Villeneuve‐d'Ascq</settlement></placeName></affiliation></author><author><name sortKey="Porchet Ennere, Eliane" sort="Porchet Ennere, Eliane" uniqKey="Porchet Ennere E" first="Eliane" last="Porchet‐Hennere">Eliane Porchet‐Hennere</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Endocrinologie des Invertébrés, CNRS UA 148, Université de Lille‐Flandres‐Artois, F‐59655 Villeneuve‐d'Ascq Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Hauts-de-France</region><region type="old region" nuts="2">Nord-Pas-de-Calais</region><settlement type="city">Villeneuve‐d'Ascq</settlement></placeName></affiliation></author><author><name sortKey="Curgy, Jean Acques" sort="Curgy, Jean Acques" uniqKey="Curgy J" first="Jean‐Jacques" last="Curgy">Jean‐Jacques Curgy</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Endocrinologie des Invertébrés, CNRS UA 148, Université de Lille‐Flandres‐Artois, F‐59655 Villeneuve‐d'Ascq Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Hauts-de-France</region><region type="old region" nuts="2">Nord-Pas-de-Calais</region><settlement type="city">Villeneuve‐d'Ascq</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Biology of the Cell</title><idno type="ISSN">0248-4900</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1989">1989</date><biblScope unit="vol">67</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="167">167</biblScope><biblScope unit="page" to="171">171</biblScope></imprint><idno type="ISSN">0248-4900</idno></series><idno type="istex">672C54E5C41ADEBAA864BDF4D175704F7C08B5B0</idno><idno type="DOI">10.1111/j.1768-322X.1989.tb00859.x</idno><idno type="ArticleID">BOC859</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0248-4900</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Polychaeta</term><term>cadmium‐binding protein</term><term>coelomocyte</term><term>in vitro and in vivo translation</term><term>mRNA</term><term>metalloprotein</term><term>monoclonal anti‐MP II antibodies</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The presence in the marine worm Nereis diversicolor of a low molecular mass protein with the capacity to bind cadmium has been previously demonstrated [5]. Poly(A)+‐mRNA were extracted from coelomocytes of Nereis diversicolor and were translated either in vitro, using a rabbit reticulocyte lysate, or in vivo into Xenopus laevis oocytes. Analysis of synthesized polypeptides by enzyme‐linked immunosorbent assay (ELISA) and by Western blotting, using a specific monoclonal anti‐MP II antibody, showed that this metalloprotein was translated both in in vitro and in vivo translation systems, with an apparent molecular mass of 11–13 kDa. Two other products, with 26.5 and 28 kDa molecular mass, cross‐reacted with the monoclonal anti‐MP II antibodies. The present work confirms that coelomocytes are sites of important synthesis of MP II‐mRNA.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Hauts-de-France</li><li>Nord-Pas-de-Calais</li></region><settlement><li>Villeneuve‐d'Ascq</li></settlement></list><tree><country name="France"><region name="Hauts-de-France"><name sortKey="Benouareth, Djamal Eddine" sort="Benouareth, Djamal Eddine" uniqKey="Benouareth D" first="Djamal Eddine" last="Benouareth">Djamal Eddine Benouareth</name></region><name sortKey="Curgy, Jean Acques" sort="Curgy, Jean Acques" uniqKey="Curgy J" first="Jean‐Jacques" last="Curgy">Jean‐Jacques Curgy</name><name sortKey="Dhainaut Ourtois, Nicole" sort="Dhainaut Ourtois, Nicole" uniqKey="Dhainaut Ourtois N" first="Nicole" last="Dhainaut‐Courtois">Nicole Dhainaut‐Courtois</name><name sortKey="Porchet Ennere, Eliane" sort="Porchet Ennere, Eliane" uniqKey="Porchet Ennere E" first="Eliane" last="Porchet‐Hennere">Eliane Porchet‐Hennere</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/CadmiumV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B75 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000B75 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Terre
|area= CadmiumV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:672C54E5C41ADEBAA864BDF4D175704F7C08B5B0
|texte= Translation in a cell‐free system, and in Xenopus oocytes, of mRNA which specify a cadmium‐binding protein in Nereis diversicolor (annelida, polychaeta)
}}
| This area was generated with Dilib version V0.6.38. |  |